原　博　教授
Hiroshi Hara

■所属学科：食物栄養学科
■メールアドレス： hara@fujijoshi.ac.jp
■研究室： 259室
■オフィスアワー： 月曜日13時～16時

農芸化学
栄養生化学

　栄養素を中心とした食品成分の健康増進／疾病予防作用を研究しています。特に、消化管における食品成分間の相互作用に力を入れており、例えば、食物繊維やオリゴ糖によるフラボノイド類やミネラル類の吸収促進作用は、これら食品中微量成分の機能性発揮に重要な意味を持つと考えています。

　学生以来、ずっと基礎栄養学分野で学び、研究してきました。この分野は、まだまだ未知の部分が沢山ありとても魅力的です。栄養素をはじめとする食品成分の生理作用を基礎から学び、氾濫する食情報を正しく理解する力を身につけるために学びましょう。

栄養素の役割や病態との関連を、分子や細胞のレベルで学びます。体全体の代謝を見据えつつ、体内で起こっている分子の世界を学びます。

人体の成り立ち、および食物を摂取した際の体の反応、さらには生体の恒常性やその破綻による慢性代謝病を理解する上での基本となる、生体成分の構造と栄養素の代謝を学びます。

生体内で起きている複雑な代謝経路や調節機構を理解するため、様々な実験を行います。そして、摂取した食事による栄養状態の変化が、身体指標がどのように影響するのかを学びます。

?H. Hara, J.M. Gee, I.T. Johnson: Antiproliferative effects of quercetin in the rat gastrointestinal tract. COST Action 98 Effects of antinutrients on the nutritional value of legume diets Vol. 8, Europian Commission (2000).

?原　博：腸内発酵（大腸発酵）「醗酵ハンドブック」共立出版、東京 (2001)

?原　博、西　隆司、比良　徹：II．消化吸収細胞実験法　３上皮細胞機能の実験法　(b)腸管内分泌細胞機能測定「生物化学実験法50腸管細胞機能実験法、上野川修一?清水　誠?八村敏志?戸塚　護 編著」学会出版センター (2005)

?原　博：機能性多糖と少糖—オリゴ糖「食品機能性の科学」産業技術サービスセンター、東京 (2008)

?原　博：ミネラル吸収促進作用「食物繊維：基礎と応用」日本食物繊維学会監修?編集、
第一出版、東京 (2008)

?原　博：食欲抑制ペプチド「大豆のすべて」喜多村啓介　編集委員長　SCIENCE FORUM、東京 (2010)

?原　博：消化管上皮のタイトジャンクションを制御する難消化性糖質 「ルミナコイド研究のフロンティア—食物繊維?オリゴ糖?レジスタントスターチの最新研究動向」　pp. 32-58, 日本栄養?食糧学会 監修、建帛社、東京 (2010)

?原　博、比良　徹：消化管内分泌系を介して作用する機能性ペプチド 「機能性タンパク質?ペプチドと生体利用」pp. 127-150, 日本栄養?食糧学会 監修、建帛社、東京 (2010/06)

?Ryouta Maeba, Hiroshi Hara: Serum choline plasmalogen is a reliable biomarker for atherogenic status. “Coronary Artery Disease - New Insights and Novel Approaches, Chapter 12, pp.243-260, InTech - Open Access Publisher (2012).

?原　博：第2章 タンパク質?ペプチド 5. ポリグルタミン酸 pp. 105-108「食品機能性成分の吸収?代謝機構」監修 宮澤 陽夫、シーエムシー出版、東京 (2013)

?原　博：第2章 タンパク質?ペプチド 1. 概観：タンパク質?ペプチドの消化?吸収?代謝?生理作用 pp. 67-77「食品機能性成分の吸収?代謝機構」監修 宮澤 陽夫、シーエムシー出版、東京 (2013)

?原　博：第1章値消化性オリゴ糖によるフラボノイド生理作用の増強「食品因子による栄養機能制御」pp. 15-27 日本栄養?食糧学会 監修、芦田　均、立花宏文、原　博 責任編集、建帛社、東京 (2015)

Nakano, K. and Hara, H. (1979) Insulin dependent and independent actions of dietary-protein on invitro protein-synthesis in skeletal-muscle of rats. Journal of Nutrition. 109, 1390-1398.

Hara, H.,Funabiki,R., Iwata, M. and Yamazaki, K. (1984) Portal absorption of small peptides in rats under unrestrained conditions. Journal of Nutrition. 114, 1122-1129.

Hara, H. and Kiriyama, S. (1991) Absorptive behavior of oligo-l-methionine and dietary proteins in a casein or soybean protein-diet - Porto-venous differences in amino-acid-concentrations in unrestrained rats. Journal of Nutrition. 121, 638-645.

Hara, H., Nishikawa, H. and Kiriyama, S. (1992) Different effects of casein and soybean protein on gastric-emptying of protein and small intestinal transit after spontaneous feeding of diets in rats. British Journal of Nutrition. 68, 59-66.

Hara, H., Fujibayashi, A. and Kiriyama, S. (1992) Pancreatic protease secretion profiles after spontaneous feeding of casein or soybean protein-diet in unrestrained conscious rats. Journal of Nutritional Biochemistry. 3, 176-181.

Hara, H.,Saito, Y., Nagata, M.,Tsuji, M., Yamamoto, K. and Kiriyama, S. (1994) Artificial fiber complexes composed of cellulose and guar gum or psyllium may be better sources of soluble fiber for rats than comparable fiber mixtures. Journal of Nutrition. 124, 1238-1247.

Hara, H., Narakino, H. and Kiriyama, S. (1994) Enhancement of pancreatic-secretion by dietary-protein in rats with chronic diversion of bile pancreatic-juice from the proximal small-intestine. Pancreas. 9, 275-279.

Hara, H., Nishi, T. and Kasai, T. (1995) A protein less sensitive to trypsin, guanidinated casein, is a potent stimulator of exocrine pancreas in rats. Proceedings of the Society For Experimental Biology and Medicine. 210, 278-284.

Hara, H., Miyashita, K., Ito, S. and Kasai, T. (1996) Oxidized ethyl linoleate induces mucosal hypertrophy of the large intestine and affects cecal fermentation of dietary fiber in rats. Journal of Nutrition. 126, 800-806.

Hara, H., Nagata, M., Ohta, A. and Kasai, T. (1996) Increases in calcium absorption with ingestion of soluble dietary fibre, guar-gum hydrolysate, depend on the caecum in partially nephrectomized and normal rats. British Journal of Nutrition. 76, 773-784.

Hara, H., Nishi, T., Narakino, H. and Kasai, T. (1996) CCK-independent increases in pancreatic secretion induced by dietary protein in chronic BPJ-diverted rats. American Journal of Physiology-Gastrointestinal and Liver Physiology. 271, G501-G508.

Hara, H., Haga, S., Kasai, T. and Kiriyama, S. (1998) Fermentation products of sugar-beet fiber by cecal bacteria lower plasma cholesterol concentration in rats. Journal of Nutrition. 128, 688-693.

Ohta, A., Ohtsuki, M., Uehara, M., Hosono, A., Hirayama, M., Adachi, T. and Hara, H. (1998) Dietary fructooligosaccharides prevent postgastrectomy anemia and osteopenia in rats. Journal of Nutrition. 128, 485-490.

Suzuki, T., Hara, H., Kasai, T. and Tomita, F. (1998) Effects of difructose anhydride III on calcium absorption in small and large intestines of rats. Bioscience Biotechnology and Biochemistry. 62, 837-841.

Hara, H., Haga, S., Aoyama, Y. and Kiriyama, S. (1999) Short-chain fatty acids suppress cholesterol synthesis in rat liver and intestine. Journal of Nutrition. 129, 942-948.

Hara, H., Konishi, A. and Kasai, T. (2000) Contribution of the cecum and colon to zinc absorption in rats. Journal of Nutrition. 130, 83-89.

Hara, H., Ohyama, S. and Hira, T. (2000) Luminal dietary protein, not amino acids, induces pancreatic protease via CCK in pancreaticobiliary-diverted rats. American Journal of Physiology-Gastrointestinal and Liver Physiology. 278, G937-G945.

Hara, H., Suzuki, T. and Aoyama, Y. (2000) Ingestion of the soluble dietary fibre, polydextrose, increases calcium absorption and bone mineralization in normal and total-gastrectomized rats. British Journal of Nutrition. 84, 655-661.

Hayashi, K., Hara, H., Asvarujanon, P., Aoyama, Y. and Luangpituksa, P. (2001) Ingestion of insoluble dietary fibre increased zinc and iron absorption and restored growth rate and zinc absorption suppressed by dietary phytate in rats. British Journal of Nutrition. 86, 443-451.

Mineo, H., Hara, H., Kikuchi, H., Sakurai, H. and Tomita, F. (2001) Various indigestible saccharides enhance net calcium transport from the epithelium of the small and large intestine of rats in vitro. Journal of Nutrition. 131, 3243-3246.

Shiga, K., Hara, H., Suzuki, T., Nishimukai, M., Konishi, A. and Aoyama, Y. (2001) Massive large bowel resection decreases bone strength and magnesium content but not calcium content of the femur in rats. Nutrition. 17, 397-402.

Mineo, H., Hara, H., Shigematsu, N., Okuhara, Y. and Tomita, F. (2002) Melibiose, difructose anhydride III and difructose anhydride IV enhance net calcium absorption in rat small and large intestinal epithelium by increasing the passage of tight junctions in vitro. Journal of Nutrition. 132, 3394-3399.

Mitamura, R., Hara, H., Aoyama, Y. and Chiji, H. (2002) Supplemental feeding of difructose anhydride III restores calcium absorption impaired by ovariectomy in rats. Journal of Nutrition. 132, 3387-3393.

Afsana, K., Shiga, K., Ishizuka, S. and Hara, H. (2003) Ingestion of an indigestible saccharide, difructose anhydride III, partially prevents the tannic acid-induced suppression of iron absorption in rats. J Nutr. 133, 3553-3560.

Hara, H., Wakisaka, T. and Aoyama, Y. (2003) Lymphatic absorption of plasmalogen in rats. British Journal of Nutrition. 90, 29-32.

Nishi, T., Hara, H., Asano, K. and Tomita, F. (2003) The soybean beta-conglycinin beta 51-63 fragment suppresses appetite by stimulating cholecystokinin release in rats. Journal of Nutrition. 133, 2537-2542.

Nishi, T., Hara, H. and Tomita, F. (2003) Soybean beta-conglycinin peptone suppresses food intake and gastric emptying by increasing plasma cholecystokinin levels in rats. Journal of Nutrition. 133, 352-357.

Nishimukai, M., Wakisaka, T. and Hara, H. (2003) Ingestion of plasmalogen markedly increased plasmalogen levels of blood plasma in rats. Lipids. 38, 1227-1235.

Hara, H. and Shiota, H. (2004) Differential increases in syntheses of newly identified trypsinogen 2 isoforms by dietary protein in rat pancreas. Experimental Biology and Medicine. 229, 772-780.

Matsumoto, M., Chiji, H. and Hara, H. (2005) Intestinal absorption and metabolism of a soluble flavonoid, alpha G-rutin, in portal cannulated rats. Free Radical Research. 39, 1139-1146.

Mitamura, R. and Hara, H. (2006) Ingestion of difructose anhydride III partially restores calcium absorption impaired by vitamin D and estrogen deficiency in rats. European Journal of Nutrition. 45, 242-249.

Tamura, A., Nishimukai, M., Shigematsu, N. and Hara, H. (2006) Supplementation of difructose anhydride III enhanced elevation of plasma equol concentrations and lowered plasma total cholesterol in isoflavone-fed rats. British Journal of Nutrition. 96, 442-449.

Bin Sufian, M. K. N., Hira, T., Asano, K. and Hara, H. (2007) Peptides derived from dolicholin, a phaseolin-like protein in country beans (Dolichos lablab), potently stimulate cholecystokinin secretion from Enteroendocrine STC-1 cells. Journal of Agricultural and Food Chemistry. 55, 8980-8986.

Suzuki, T., Yoshida, S. and Hara, H. (2008) Physiological concentrations of short-chain fatty acids immediately suppress colonic epithelial permeability. British Journal of Nutrition. 100, 297-305.

Hira, T., Mochida, T., Miyashita, K. and Hara, H. (2009) GLP-1 secretion is enhanced directly in the ileum but indirectly in the duodenum by a newly identified potent stimulator, zein hydrolysate, in rats. American Journal of Physiology-Gastrointestinal and Liver Physiology. 297, G663-G671.

Matsukawa, N., Matsumoto, M., Shinoki, A., Hagio, M., Inoue, R. and Hara, H. (2009) Nondigestible Saccharides Suppress the Bacterial Degradation of Quercetin Aglycone in the Large Intestine and Enhance the Bioavailability of Quercetin Glucoside in Rats. Journal of Agricultural and Food Chemistry. 57, 9462-9468.

Hara, H., Onoshima, S. and Nakagawa, C. (2010) Difructose anhydride III promotes iron absorption in the rat large intestine. Nutrition. 26, 120-127.
Mochida, T., Hira, T. and Hara, H. (2010) The Corn Protein, Zein Hydrolysate, Administered into the Ileum Attenuates Hyperglycemia via Its Dual Action on Glucagon-Like Peptide-1 Secretion and Dipeptidyl Peptidase-IV Activity in Rats. Endocrinology. 151, 3095-3104.

Nakajima, S., Hira, T., Eto, Y., Asano, K. and Hara, H. (2010) Soybean beta 51-63 peptide stimulates cholecystokinin secretion via a calcium-sensing receptor in enteroendocrine STC-1 cells. Regulatory Peptides. 159, 148-155.

Shinoki, A. and Hara, H. (2010) Calcium deficiency in the early stages after weaning is associated with the enhancement of a low level of adrenaline-stimulated lipolysis and reduction of adiponectin release in isolated rat mesenteric adipocytes. Metabolism-Clinical and Experimental. 59, 951-958.

Hira, T., Mori, N., Nakamori, T., Furuta, H., Asano, K., Chiba, H. and Hara, H. (2011) Acute effect of soybean beta-conglycinin hydrolysate ingestion on appetite sensations in healthy humans. Appetite. 57, 765-768.

Shinoki, A. and Hara, H. (2011) Dietary fructo-oligosaccharides improve insulin sensitivity along with the suppression of adipocytokine secretion from mesenteric fat cells in rats. British Journal of Nutrition. 106, 1190-1197.

Suzuki, T. and Hara, H. (2011) Role of flavonoids in intestinal tight junction regulation. Journal of Nutritional Biochemistry. 22, 401-408.

Higuchi, N., Hira, T., Yamada, N. and Hara, H. (2013) Oral administration of corn zein hydrolysate stimulates GLP-1 and GIP secretion and improves glucose tolerance in male normal rats and Goto-Kakizaki rats. Endocrinology. 154, 3089-3098.

Shinoki, A., Lang, W., Thawornkuno, C., Kang, H. K., Kumagai, Y., Okuyama, M., Mori, H., Kimura, A., Ishizuka, S. and Hara, H. (2013) A novel mechanism for the promotion of quercetin glycoside absorption by megalo α-1,6-glucosaccharide in the rat small intestine. Food Chem. 136, 293-296.

Nishimukai, M., Maeba, R., Ikuta, A., Asakawa, N., Kamiya, K., Yamada, S., Yokota, T., Sakakibara, M., Tsutsui, H., Sakurai, T., Takahashi, Y., Hui, S. P., Chiba, H., Okazaki, T. and Hara, H. (2014) Serum choline plasmalogens-those with oleic acid in sn-2-are biomarkers for coronary artery disease. Clin Chim Acta. 437, 147-154.

Phuwamongkolwiwat, P., Suzuki, T., Hira, T. and Hara, H. (2014) Fructooligosaccharide augments benefits of quercetin-3-O-β-glucoside on insulin sensitivity and plasma total cholesterol with promotion of flavonoid absorption in sucrose-fed rats. Eur J Nutr. 53, 457-468.

Yuki Ishikawa, Tohru Hira, Daisuke Inoue, Yukikazu Harada, Hiroyuki Hashimoto, Mikio Fujii, Motoni Kadowaki, Hiroshi Hara (2015) Rice protein hydrolysates stimulate GLP-1 secretion, reduce GLP-1 degradation, and lower the glycemic response in rats. Food & Function 6, 2525-2534.

Tohru Hira, Asuka Yahagi, Saki Nishimura, Masayoshi Sakaino, Takatoshi Yamashita, Hiroshi Hara (2015) Diunsaturated aldehyde, trans,trans-2,4-decadienal in the intestinal lumen suppresses gastric emptying through serotonin signaling in rats. Journal of Agricultural and Food Chemistry 63, 8177-81.

Tanaka Seiya, Shinoki Aki, Hara Hiroshi (2016) Melibiose, a Nondigestible Disaccharide, Promotes Absorption of Quercetin Glycosides in Rat Small Intestine. J Agric Food Chem. 64(49):9335-9341.

Hiroshi Hara, Shunsuke Kume, Takahisa Iizuka, Yoshinori Fujimoto & Atsuo Kimura  (2017) Enzymatically synthesized megalo-type isomaltosaccharides enhance the barrier function of the tight junction in the intestinal epithelium. Bioscience, Biotechnology, and Biochemistry.82(4):629-635.

Yusaku Iwasaki, Mio Sendo, Katsuya Dezaki, Tohru Hira, Takehiro Sato, Masanori Nakata, Chayon Goswami, Ryohei Aoki, Takeshi Arai, Parmila Kumari, Masaki Hayakawa, Chiaki Masuda, Takashi Okada, Hiroshi Hara, Daniel J. Drucker, Yuichiro Yamada, Masaaki Tokuda, Toshihiko Yada (2018) GLP-1 release and vagal afferent activation mediate the beneficial metabolic and chronotherapeutic effects of D-allulose. Nature Communication 9(1):113.

Seiya Tanaka, Aphichat Trakooncharoenvit, Miyu Nishikawa, Shinichi Ikushiro and Hiroshi Hara (2019) Comprehensive analyses of quercetin conjugates by LC/MS/MS revealed that isorhamnetin-7-O-glucuronide-4’-O-sulfate is a major metabolite in plasma of rats fed with quercetin glucosides. J Agric Food Chem. 67(15):4240-4249.